Journal: Heliyon

Article Title: Technical considerations regarding saliva sample collection to achieve comparable protein identification and detection via one- and two-dimensional gel electrophoresis among humans

doi: 10.1016/j.heliyon.2024.e40752

Figure Lengend Snippet: Utilization of Bio-Rad ReadyPrep 2-D Cleanup kit™ and ReadyPrep Reduction-Alkylation kit™ on native human saliva samples alter protein yield. Representaive 2D gel electrophoresis results of untreated ( a ) and ReadyPrep 2-D Cleanup kit™, ReadyPrep Reduction-Alkylation kit™ treated ( b ) native human saliva samples (no RNAlater™ treatment) demonstrate both procedures alter protein yield primarily at the low molecular range. Highlighted areas (1–3) indicate identical regions of the separation. Proteins were visualized by silver stain.

Article Snippet: Urea, glycerol and mineral oil were obtained from Sigma, thiourea, sodium thiosulfate, bromophenol blue, glycine from Reanal, 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS), Bio-Lyte, dithiothreitol, iodoacetamide, Silver Stain Plus Kit and Bio-Safe Coomassie G-250 Stain from Bio-Rad, and SDS from Serva.

Techniques: Two-Dimensional Gel Electrophoresis, Electrophoresis, Silver Staining